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KMID : 0381120070290040503
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Genes and Genomics
2007 Volume.29 No. 4 p.503 ~ p.511
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Molecular and Cytological Characterization of Species-Specific Repetitive DNA Sequences for Angelica acutiloba
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Choi Hae-Woon
Song Ha-Young
Koo Dal-Hoe
Bang Jae-Wook
Hur Yoon-Kang
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Abstract
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In order to identify and discriminate a medicinal herb, A. acutiloba, from closely related species, species-specific repetitive DNAs have been cloned and characterized by Southern blotting and FISH (fluorescence in situ hybridization). From restriction enzyme analysis, tandemly repeated sequences specific for A. acutiloba (AaTRS) were obtained from an EcoRI digestion. The EcoRI monomers were 154-159 bp long, 60-66% of A+T residues and did not show any similarity to the known plant repetitive DNAs. Its repeats are organized in head-to-tail orientation. Southern blot analysis of genomic DNA digested with the same enzyme showed the existence of a ladder pattern only in A. acutiloba, indicating that the
repetitive DNA is arrayed in tandem. In FISH experiment, the AaTRS was localized on the telomeric regions of somatic metaphase chromosomes in A. acutiloba. Although seven monomers revealed high sequence variation as the typical features of eukaryotic repetitive DNA, SCAR (sequence characterized amplified region) markers generated from their sequence information could discriminate A. acutiloba from two other species.
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KEYWORD
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Angelica acutiloba, tandemly repeated DNA sequence, SCAR marker, telomeric, FISH
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